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For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server: HOTGpred has been established. Moreover, to maximize users' convenience, a step-by-step guide is given below on how to use the web-server and get the desired results without the need to through the mathematical details of the algorithm.

• Step 1
  Go to the HOTGpred homepage by copying the following link https://balalab-skku.org/HOTGpred/ to the web browser or click here, and you will see its top page of HOTGpred. Click on the Help button to see a brief introduction about the server (this page).

• Step 2
  Paste your query peptide sequences into the input box or directly upload your input file via the "Choose File" button. The input sequences must be in the FASTA format otherwise the program will throw an error. We have provided a set of sample FASTA sequences. Users can click (Example) for easy testing of the HOTGpred server.

• Step 5
  Click on the Reset/clear button if you need to erase the query sequences present in the input box or file upload button.

• Step 6
  Click on the Download button to download datasets (training and independent) used in this paper.