For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server: MeL-STPhos has been established. Moreover, to maximize users' convenience, a step-by-step guide is given below on how to use the web-server and get the desired results without the need to through the mathematical details of the algorithm.
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Step 1Go to the MeL-STPhos homepage by copying the following link https://balalab-skku.org/MeL-STPhos/ to the web browser or click here, and you will see its top page of MeL-STPhos. Click on the Help button to see a brief introduction about the server (this page).
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Step 2We have developed the MeL-STPhos phosphorylation-site-specific prediction model for three different phosphorylation sites. Therefore, users can select an appropriate phosphorylation sites for the prediction using the dropdown menu.
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Step 3Paste your query protein sequences into the input box or directly upload your input file via the "Choose File" button. The input sequences must be in the FASTA format otherwise the program will throw an error. We have provided a set of sample FASTA sequences. Users can click (Example) for easy testing of the MeL-STPhos server.
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Step 4By clicking on the submit button, you will get the prediction results on your computer screen.
- For the selected phosphorylation-site-specific model, four columns are displayed. The first, the second, the third, and the fourth columns represent serial number, protein id (i.e. user FASTA ID), Phosphorylated or Non-phosphorylated and probability of prediction.
- Notably, the probability score ranges from 0 to 1, where a score close to 0 or 1 represents Non-phosphorylated or Phosphorylated, respectively.
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Step 5Click on the Reset/Clear button if you need to erase the query sequences present in the input box or file upload button.
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Step 6Click on the Download button to download datasets (training and independent) used in this paper.
