For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server: TACOS has been established. Moreover, to maximize users' convenience, a step-by-step guide on how to use the web-server and get the desired results is given below without the need to go through the mathematical details of the algorithm.
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Step 1Go to the TACOS homepage by copying the following link https://balalab-skku.org/tacos/ to the web browser. Click on the Help button to see a brief instruction of how to use the prediction server (this page).
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Step 2Paste your query RNA sequences into the input box or directly upload your input file via the "Choose File" button. The input sequences must be in the FASTA format otherwise the program will throw an error. We have provided a set of sample FASTA sequences (Click Example) for easy testing of the TACOS server.
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Step 3We have developed the TACOS Cell-Specific prediction model for ten different cell types. Therefore, users can select an appropriate Cell-type for the prediction using the dropdown menu.
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Step 4By clicking on the submit button, you will get the prediction results on your computer screen.
- For the selected cell-specific model, four columns are displayed. The first, second, third, and fourth columns represent serial number, sequence identifier, subcellular localization, and prediction probability, respectively..
- Notably, the probability score of subcellular localization ranges from 0 to 1, where a score close to 0 or 1 represents nucleus or cytoplasm, respectively.
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Step 5Click on the Reset/clear button if you need to erase the query sequences present in the input box or file upload button.
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Step 6Click on the Download button to download datasets (training and independent) used in this paper.