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For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server: ac4C-AFL has been established. Moreover, to maximize users' convenience, a step-by-step guide is given below on how to use the web-server and get the desired results without the need to through the mathematical details of the algorithm.

• Step 1
  Go to the ac4C-AFL homepage by copying the following link https://balalab-skku.org/ac4C-AFL/ to the web browser or click here, and you will see its top page of ac4C-AFL. Click on the Help button to see a brief introduction about the server (this page).

• Step 2
  Paste your query RNA sequences into the input box or directly upload your input file via the "Browse" button. The input sequences must be in the FASTA format otherwise the program will throw an error. We have provided a set of sample FASTA sequences. Users can click (Example) for easy testing of the ac4C-AFL server.

• Step 3
  By clicking on the submit button, you will get the prediction results on your computer screen.
  • The first, the second, the third, and the fourth columns represent serial number, RNA ID (i.e. user FASTA ID), ac4C or non-ac4C and probability of prediction.
  • Notably, the probability score ranges from 0 to 1, where a score close to 0 or 1 represents non-ac4C or ac4C, respectively.

• Step 4
  Click on the Reset/Clear button if you need to erase the query sequences present in the input box or file upload button.

• Step 5
  Click on the Download button to download datasets (training and independent) used in this paper.