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For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server: MLCPP 2.0 has been established. Moreover, to maximize users' convenience, a step-by-step guide is given below on how to use the web-server and get the desired results without the need to through the mathematical details of the algorithm.

• Step 1
  Copy the following link https://balalab-skku.org/mlcpp2/ to the web browser or click here, and you will see its top page of MLCPP 2.0. Click on the Help button to see a brief introduction about the server.

• Step 2
  Enter the query protein/peptide sequences into the input box at the center . You can also directly upload input file via the 'Choose File' option. The input sequences should be in the FASTA format. Examples of FASTA-formatted sequences can be seen by clicking on the Example button right above the input box.

• Step 3
  By clicking on the button submit, you will get the predicted results on the screen of your computer. The first, the second, the third and the fourth column respectively represent serial number, peptide id (i.e. user FASTA ID), Cell-penetrating or not and probability of CPP. If a given peptide is predicted as CPP, the fifth and the sixth column respectively represent uptake efficiency and probability of high uptake efficiency, otherwise “ — “.

• Step 4
  Click on the Reset button to reload the page or click the Clear button to clear the previous sequences or file present in the input box or the file upload box.

• Step 4
  Click on the Dataset button to download the datasets (training and independent) used in this paper.